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Enzyme assay answers (110)

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Q: 

enzyme assay

A: There are two common methods of determining the activity of an enzyme. A stop time assay and a real-time assay. A stop time assay is just that, start the reaction and stop or read the results at a given time. This is the easiest way to do many assays at one time BUT there are two things that need to be considered before doing this form of the assay. First is the assay linear. In other words, in the time that I am running the assay, is the product being produced (or substrate converted) at a linear rate. If the conditions of the assay tube are such that...
Q: 

Is 1.98 Result From G6pd Confirmatory enzyme assay Negative Or Positive?

A: Normal levels of G6PD levels are 8-8.6 units/gram of hemoglobin. You are having G6PD deficiency. For details, visit this link.. ...


Q: 

What Is Involved in Measuring enzyme Levels?

A: Laboratory tests that measure enzyme levels and enzyme activity are known as enzyme assays. There are many types of measurement methods that measure the rate of enzyme activities and enzyme inhibitions. enzymes are molecules that manipulate other molecules, known as substrates, by binding with them and chemically reacting with them to produce multiple by-products. The tests that measure this activity are for differing purposes, according to particular disease symptoms, and regard differing steps in enzymatic processes.. assays that examine enzyme kinetics show how much...


Q: 

What Is an enzyme-Linked Immunosorbent assay?

A: An enzyme-linked immunosorbent assay (ELISA) is a type of test that is used to measure antibodies or antigens in a fluid sample. Also known as an enzyme immunoassay (EIA) test, an ELISA is a biochemical technique that is commonly used in immunology. It is used to diagnose conditions that may trigger an immune system response. enzymes are used to tag antigens or antibodies to determine the type of disease, illness, or allergy a person has.. Blood tests are beneficial aids in diagnosing many different problems. Diseases such as cancer and autoimmune conditions can be detected with an enzyme-linked immunosorbent assay....


Q: 

What Is an Ubiquitination assay?

A: Ubiquitination refers to the binding of the protein ubiquitin to other proteins by three different enzymes. A ubiquitination assay is a test that determines how much of this process occurs within the cell. Tests can be used to determine how different conditions affect protein binding, steps and structures that are important in the ubiquitinnation pathway, and the amount of protein in the cell. Inadequate or overabundant amounts have been linked to the development of many different diseases.. A protein found in cells, the function of ubiquitin is to bind to proteins while functioning as a "tag" or signal to other structures. This "tag" then signals enzymes to know that the protein can be destroyed, recycled, or...


Q: 

cell bio

A: Cassie, Wow! what a complicated question! This information will be found in any basic biochemistry textbook, so you might want to start there. I"m thinking that the first part of the question wants something like "enzyme activity is measured directly by measuring the catalysis of the specific substrate for the enzyme, and indirectly by measuring a downstream product of the enzyme catalysis"  You could find some specific example for both types of assay, perhaps beta-galactosidase which cleaves x-galactoside to a blue-colored product (direct), or cytochrome oxidase in the mitochondria that helps to produce ATP (indirect). For kinetic data collection, generally it is a timed...


Q: 

what is tays sach choroiditis?

A: The condition is caused by insufficient activity of an enzyme called hexosaminidase A that catalyzes the biodegradation of fatty acid derivatives known as gangliosides. Hexasaminidase A is a vital hydrolytic enzyme , found in the lysosomes, that breaks down lipids. When Hexasaminidase A is no longer functioning properly, the lipids accumulate in the brain and cause problems. Gangliosides are made and biodegraded rapidly in early life as the brain develops. Patients and carriers of Tay-Sachs disease can be identified by a simple blood test that measures hexosaminidase A activity. TSD is a recessive genetic disorder, meaning that both parents must be carriers in order to give birth to an affected child. Even then, there is only a 25% chance...
Q: 

Why the drug is not given by oral route uptill now?

A: can you please specify which drug do you mean. Some of the medications can not be used orally because are inactivated by acid medium in the stomach. dear smith, thanks for the responce, i want to know about the drug azelastine hydrochloride detail pharmacokinetics (ADME). JohnySmithPharmacokinetics and Metabolism After intranasal administration, the systemic bioavailability of azelastine hydrochloride is approximately 40%. Maximum plasma concentrations (Cmax) are achieved in 2-3 hours. Based on intravenous and oral administration, the elimination half-life, steady-state volume of distribution, and plasma clearance are 22 hours, 14.5 L/kg, and 0.5 L/h/kg, respectively. Approximately 75% of an oral dose of radiolabeled azelastine hydrochloride was excreted in the feces with less than 10% as...


Q: 

Your answer to a question about falling CD4''s despite undetectable VL

A: Response from Dr. Lee To respond to your first question, the following is my answer from a question in switch/simplify dated Jan 15 04 on ''Low VL'': You mentioned that you are from a third world country, although you did not specify which one. As you may know there are different families (clades) of HIV which are predominent in different areas of the world. In the Northwest Hemisphere (US and Canada), Australia and Western Europe the Clade B virus is the most common type of HIV. Therefore in those parts of the world, the viral load tests mostly measure Clade B viruses and may miss or undercount viruses from other clades. The practical application of this is that people who have non-B clades of HIV cannot rely on the viral load numbers which come from tests for Clade B viruses. The tests...


Q: 

Prescription probiotics to treat C. diff?

A: Hi, Indeed, it may be fair to say that your stool culture grew an "unknown" or "unidentified" strain of Clostridium difficile (C. difficile). It occurs ''normally'' in about 3-5% of healthy adults, and is common in babies and infants. Also, studies have shown that it is widely prevalent in the environment. There are two types of clostridium difficile associated diarrhea (CDAD), - hospital-aquired (nosocomial) & - community-aquired. An "Hypervirulant" strain has been found to be increasingly causing epidemics of CDAD worldwide. Isolation of C. difficile from stool samples is important from the point of view of the epidemiological typing of strains. In a given population, among the "carriers" of C. difficile there may be very few "carriers"...


 
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